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CRISPR & Gene Editing
Advances in genome engineering are enabling researchers to achieve unprecedented breakthroughs, from curing genetic disorders to developing disease-resistant crops. Sigma-Aldrich® Advanced Genomics delivers complete support for every step of CRISPR-based gene editing research at any scale. Target a single gene using Zinc Finger Nucleases or screen a whole genome with fully customizable CRISPR products. With over 350 years of technical leadership, Sigma-Aldrich® Advanced Genomics products come with expertise that extends Beyond the Bench - from discovery to therapeutic impact. Best in Class Cas9 ProteinsThe Ultimate Suite of Genome Engineering Tools and Services
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Guaranteed CRISPRgRNA
CRISPR Inhibition and Activation (CRISPRiand CRISPRa)
CRISPR Screening with 10x Genomics Compatibility
We’re Here to Help
The Sigma-Aldrich® Advanced Genomics portfolio delivers the ultimate suite of genome engineering tools and services to unlock your research potential. Whether you are looking to knockout, knock-in, knockdown, or overexpress your targets, our comprehensive suite of CRISPR gene-editing tools and services will take your research Beyond the Bench.
Order Cas9 Proteins
Order Pre-designed gRNA
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Sigma-Aldrich® Advanced Genomics offers multiple Cas9 variants (wild-type, enhanced specificity, nickase, GFP-fused, catalytically inactive) and in several formats (plasmid, lentivirus, lyophilized protein). Choose from an industry leading selection of Cas9 proteins to meet your individual research needs.Best in Class Cas9 Proteins
Every SygRNA® synthetic single guide RNA (sgRNA), crRNA and tracrRNA is synthesized using our proprietary process and reagents using the highest quality materials available. Editing activity is assured regardless of your target, cell type or application, and is backed by the most comprehensive guarantee in the industry.Guaranteed CRISPR gRNA
Gain even greater insights into complex biological networks using Sigma-Aldrich® Advanced Genomics CRISPR activation and CRISPR inhibition libraries for gene knockdown and overexpression experiments. Pooled CRISPRi and SAM CRISPRa lentiviral libraries are available off-the-shelf or customized to your specific needs.CRISPR Inhibition and Activation (CRISPRi and CRISPRa)
Sigma-Aldrich® Advanced Genomics offers the only 10x Genomics Certified Compatible lentiviral pools for CRISPR single-cell screening. Our optimized, custom lentiviral vectors are fully compatible with Chromium Single Cell Gene Expression v3 and v3.1 (Next GEM) solution utilizing Feature Barcode technology.CRISPR Screening with 10x Genomics Compatibility
Whether you need help designing your CRISPR experiment, require assistance troubleshooting, or simply want to better understand how you can apply gene editing methods in your research, Sigma-Aldrich® Advanced Genomics is here to guide you. Wondering if CRISPR is right for your project? Connect with a Sigma-Aldrich® Advanced Genomics Expert.We’re Here to Help
Related Product Resources
- Cas9-Mediated Recombineering Enhances Metabolic Production of Kdo2-Lipid A by E. coli
In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) hom*ologous recombination (HR) vector system, which facilitates gene editing through the hom*ology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either ssDNA or dsDNA as an editing template.
- CRISPR Use License Agreement
This Product and its use are the subject of one or more of the following patents controlled by The Broad Institute, Inc. (BROAD), the Massachusetts Institute of Technology (MIT), or the President and Fellows of Harvard College (HARVARD): U.S. Patent Nos. 8,697,357; 8,771,945, and any substitutions, divisions, continuations, reissues, renewals, re-examinations or extensions, and corresponding foreign patent applications and patents.
- Ribonucleoprotein (RNP) Protocols Using Synthetic sgRNAs and Cas9 proteins
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
- Genome Editing in Plants with CRISPR/Cas9
Zinc finger nucleases (ZFNs), were introduced by Sigma-Aldrich less than 8 years ago, but in that time the technology of targeted genome editing has advanced rapidly. Most recently, the discovery of the CRISPR/Cas9 pathway has accelerated interest in this field, opening up new possibilities for research and development.
- Gene Editing 101 eBook
Learn how to understand, design and perform CRISPR experiments, as well as design gRNA, choose a Cas9 format, screen with CRISPR, use advanced CRISPR formats, and much more…
Related Product Categories
Genetic Screening
Sigma-Aldrich® Advanced Genomics has the most comprehensive portfolio of functional genomics and genetic screening tools available. Whole-genome and custom designed CRISPR, RNAi, and ORF libraries, arrayed or pooled, to meet your screening needs at any scale.
RNAi Single Clones
Genome-wide human and mouse shRNA clones from the TRC1.5 and TRC2 collections, available as plasmids or high-titer virus.
RNAi Libraries
Human and mouse lentivirus shRNA screening libraries and pools from the RNAi Consortium (TRC). Available as bacterial glycerol stocks, plasmid DNA or read-to-use lentiviral particles. MISSION® RNAi libraries are also available with a range of custom vectors, volumes, and viral titers.
Related Services
Need help with deconvolution of pooled shRNA samples after screening is complete? We provide a pooled shRNA deconvolution service that will help you identify TRC clones that are of significance in your screen.
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